Research-Development-bannersMITCON’s Technology BUSINESS INCUBATOR conducts research in various areas related to technology entrepreneurship like government funding for startups, early-stage funding for technology ventures sponsored by Department of Science and Technology (DST), Govt. of India & APCTT, New Delhi. Bio-Technology, agriculture, food processing and Pharmaceutical has been set up to boost commercialization through incubation. MITCON’s Technology BUSINESS INCUBATOR Center provides facilities for researches in Microbiology, Biofertilizers, Biopesticides, Genomics, Plant Tissue culture sale and supply center, Bioinformatics laboratories.

Our Research Projects Published in Various International Journals

Abstract:

Production of pullulanase was carried out from I. batatas, P. vulgaris, E. crassipes and P. stratiotes using A. pullulans. The fermentation reactions were carried out in minimal basal media at 28°C. E. crassipes and P. stratiotes as substrates produced more pullulanase than I. batatas and P. vulgaris. The highest enzyme production from Pistia stratiotes was of 9.3 U/ml after 72 hours of incubation at 28°C and Eichhornia crassipes showed most enzyme production of 4.65 U/ml after 48 hours of incubation at 28°C. The two novel substrates, E. crassipes and P. stratiotes, could successfully be used for the production of pullulanase and are a promising resource for large-scale production of the same.

Keywords: Eichhornia crassipes, Pistia stratiotes, Ipomoea batatas, Phaseolus vulgaris, pullulanase, Aureobasidium, Solid state fermentation

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Abstract:

Biosurfactants are amphiphilic compounds produced by various bacteria and fungi which reduce surface and interfacial tension. In this work, the biosurfactant produced by Bacillus subtilis strain  isolated from soil samples was characterized and its properties compared with commercially available chemical surfactants. Bacillus subtiliswas used for the production of biosurfactant and its activity was tested against crude vegetable oil. The crude biosurfactant was produced using four different  substrates and its emulsification activity was compared against sodium dodecyl sulphate (SDS). The results showed that the isolated bio surfactant from coconut waste showed the highest emulsification activity even more than sodium dodecyl sulphate (SDS) which is a commercial chemical surfactant. Furthermore its antimicrobial activity was checked against Staphylococcus aureus, Escherichia coli, Pseudomonas and Salmonella typhimurium. The study concludes that coconut and soyabean waste  are the most ideal substrate for biosurfactant biosynthesis, which may have potential industrial applications.

Keywords: Biosurfactant, Bacillus subtilis, Emulsification, Waste Management

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ABSTRACT:
B. Subtilis (NCIM 2063) and A. Niger (NCIM 620) were used for amylase production from E. Crassipes and P. Stratiotes. The fermentation batches were set up in minimal basal media. Maximum amylase activity was seen by Aspergillus niger on Pistia stratiotes (Day 2) and Eicchornia crassipes (Day 4) at 32°C. A. niger with a spore count of 6.4×109 spores/ml after partial purification produced 6.8 units/ml enzyme in P. stratiotes and 7.3units/ml enzyme in E. crassipes with a spore count of 3.2×109 spores/ml. B. subtilis with CFU of 4.1×109 after partial purification produced 6.239 nits/ml enzyme in P. stratiotes and 6.08 units/ml enzyme in E. crassipes with CFU of 4.6×109. The results obtained are significant as there have been no reports of production of amylase from these two plants using solid state fermentation (SSF).

Keywords: Aspergillus niger, Bacillus subtilis, Eichhornia crassipes, Pistia stratiotes, Amylase and Solid substrate

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